Journal: Malaria Journal

Article Title: An external sensing system in Plasmodium falciparum -infected erythrocytes

doi: 10.1186/s12936-016-1144-6

Figure Lengend Snippet: Treatment with different inhibitors, Calpeptin and Gambogoc Acid. a ItG early trophozoite stage pRBC were treated with/without calpeptin, a calpain inhibitor for 1 h before TNF stimulation, and the expression of PfAB4 and a 90 kDa protein reacting with AB4 antibodies measured. The 100 µM calpeptin treatment caused a block of PfAB4 degradation. b ItG early trophozoite stage pRBC were treated with/without Gambogic acid (Gam), a HSP90 inhibitor overnight before TNF stimulation. The expression of PfAB4 and a 90 kDa protein reacting with AB4 antibodies was measured. Gam 0.5, 1.0 and 1.5 µM inhibited the 90 kDa protein expression and reduced the effect on PfAB4 caused by TNF

Article Snippet: The nitrocellulose membranes were blocked by 1-hour incubation in 5 % skim milk in TST buffer (0.01 M Tris pH 8.5/0.15 M sodium chloride/0.1 % Tween 20) and washed with TST buffer briefly, then probed with different primary antibodies: anti-phospho-IκB-α (Cell signalling); anti-EXP2 mAb 7.7 (kindly provided by Brendan S Crabb); Anti-HSP90α and HSP90β (Cell signalling); Anti-GAPDH (Enzo); Anti-calcineurin (Abgent); Anti-PGK1/2 (Santa Cruz).

Techniques: Expressing, Blocking Assay